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Addgene inc
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Addgene inc
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GenScript corporation
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New England Biolabs
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Image Search Results
Journal: Frontiers in Microbiology
Article Title: Repurposing of FDA Approved Drugs Against SARS-CoV-2 Papain-Like Protease: Computational, Biochemical, and in vitro Studies
doi: 10.3389/fmicb.2022.877813
Figure Lengend Snippet: Depicts the strategy used to clone the cDNA coding for SARS-CoV-2 PLpro in pET22b vector system for bacterial expression. The PLpro gene codons were optimized, synthesized, and subcloned into the pET22b vector with MscI and BIpI restriction site. N-terminal end of the clone having a 6X His tag followed by SUMO tag consisting of PLpro autocleavage site. On the other hand, C-terminals end up having 6x His tag for purification. *Autocleavable.
Article Snippet: The cDNA sequence coding for SCoV-2 PLpro was codon-optimised for bacterial expression and was custom-synthesised in
Techniques: Plasmid Preparation, Expressing, Synthesized, Purification